Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility

doi: 10.1073/pnas.1911304116

Figure Lengend Snippet: Jph2 knockdown has no effect on Ca 2+ spark frequency, amplitude, or kinetics. ( A ) Representative confocal Ca 2+ images of pressurized (60 mmHg), Fluo-4-AM–loaded cerebral arteries treated with control or Jph2 -targeting morpholinos. Colored boxes show selected ROIs where Ca 2+ sparks occurred. (Scale bar, 10 µm.) ( B ) Representative changes in fractional fluorescence ( F / F 0 ) as a function of time for ROIs in A . The trace color corresponds to the color of the respective ROI box. ( C ) Summary data showing the Ca 2+ spark frequency (in Hertz) normalized to surface area (in Hertz per 100 square micrometers) in cerebral arteries ( n = 5 to 6 cerebral arteries/group from 4 animals), as well as the amplitude ( F / F 0 ), half-duration [half-time ( t 1/2 ), in seconds], rise time ( t 1/2 , in seconds), and decay time ( t 1/2 , in seconds) of individual Ca 2+ spark events recorded from each group ( n = 616 events for control, n = 601 events for Jph2 -targeted). There were no significant differences.

Article Snippet: Rows were successively loaded with Wes antibody diluent blocking buffer; anti-JPH2 (ab116077; Abcam) or anti–β-actin (ab8227, Abcam) primary antibodies, diluted 1:20 and 1:1,500, respectively; horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (1×; ProteinSimple); and a luminol–peroxide mix.

Techniques: Fluorescence

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of lasI gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (lasI gene products at 295 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of lasR gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (lasR gene products at 130 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of rhlI gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (rhlI gene products at 155 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of rhlR gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (rhlR gene products at 133 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of aprA gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (aprA gene products at 993 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of lasB gene. MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (lasB gene products at 153 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of plcH gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (plcH gene products at 307 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of rhlAB gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (rhlAB gene products at 151 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of toxA gene. MWM-molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (toxA gene products at 150 bp).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control

Journal: International Journal of Molecular Epidemiology and Genetics

Article Title: Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products after amplification of fliC gene. MWM-molecular weight marker (O’Range Ruler 200 bp DNA ladder, # SM0633, Fermentas), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa (fliC gene products at 1.02 kb and 1.25 kb).

Article Snippet: MWM -molecular weight marker (100 bp DNA ladder, # DL004, Geneaid), +C-PAO1 control DNA, B1-B12- different strains of P. aeruginosa ( lasB gene products at 153 bp). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7 caption a7 Agarose gel electrophoresis of PCR products after amplification of plcH gene.

Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Control